Mouse 5'-Nucleotidase/CD73 ELISA Kit ELISA试剂盒,Mouse 5'-Nucleotidase/CD73 ELISA Kit/小鼠5'-核苷酸酶/ CD73 ELISA试剂盒

2024-10-24

Mouse 5'-Nucleotidase/CD73 ELISA Kit/小鼠5'-核苷酸酶/ CD73 ELISA试剂盒

货号:EM2RB,EM2RBX5,EM2RBX10

规格:96 Tests,5X96 Tests,10X96 tests

价格:6641,29884,57271

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

7 pg/mL

检测范围7-1,500 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCD73
Protein aliases5'-NT, 5'-nucleotidase, CD73, Ecto-5'-nucleotidase

物种(Tested)

小鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliases2210401F01Rik, AI447961, CD73, eNT, NT, Nt5, Nt5e, Nte
Gene ID(Mouse) 23959
Gene symbolNt5e
UniProt ID(Mouse) Q61503
Mouse 5'-Nucleotidase/CD73 quantitates mouse 5'-Nucleotidase/CD73 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse 5'-Nucleotidase/CD73.小鼠5'-核苷酸酶/ CD73定量测定血清,血浆,上清液中的小鼠5'-核苷酸酶/ CD73。该检测试剂盒将专门识别天然和重组小鼠5'-核苷酸酶/ CD73。Target information 靶标信息CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.CD73(Ecto-5-prime-核苷酸酶,5-prime-核糖核苷酸磷酸水解酶)催化嘌呤5-prime单核苷酸在中性pH下向核苷的转化,优选的底物是AMP。 CD73由2个相同的70 kDa亚基的二聚体组成,该二聚体通过糖基磷脂酰肌醇键与质膜外部结合。 CD73用作淋巴细胞分化的标志。因此,在多种免疫缺陷疾病中会出现CD73缺陷。 5-prime核苷酸酶的其他形式存在于细胞质和溶酶体中,可以通过其底物亲和力,对二价镁离子的需求,ATP的激活和无机磷酸盐的抑制与CD73区别开来。通过免疫荧光和对一组人类x小鼠杂种的研究,CD73基因已定位于6q14-q21,其中的人类x小鼠杂种含有染色体6的片段作为易位。 CD73基因的缺陷可导致关节和动脉钙化,以及肠结核。已发现编码CD73的不同同工型的两个转录物变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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