Mouse IL-18 Uncoated ELISA Kit,Mouse IL-18 Uncoated ELISA Kit/小鼠IL-18未包被ELISA试剂盒

2024-10-24

Mouse IL-18 Uncoated ELISA Kit/小鼠IL-18未包被ELISA试剂盒

货号:88-50618-88,88-50618-77

规格:10X96 tests,20X96 tests

价格:6261,8461

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<31.2 pg/mL

检测范围31.2-2000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

20X 96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, Biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

-10X包被缓冲液:用于包被捕获抗体的缓冲液

- 检测酶:Pre-titrated Avidin-HRP浓缩液(100X)

- 5X ELISA/ELISPOT 稀释液:用于封闭、稀释检测抗体和酶

- 底物溶液:1XTMB 底物溶液

- 分析证书:特定批次说明书用于抗体和标准品稀释

- 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-76,-86)

储存温度

2-8℃

Protein nameIL-18
Protein aliasesIFN-gamma-inducing factor, IL-1 gamma, IL-18, Interferon gamma-inducing factor, interferon-gamma-inducing factor, Interleukin-1 gamma, Interleukin-18

物种(Tested)

小鼠

试剂盒类型

Uncoated ELISA Kitwith Plates

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIgif, Il-18, Il18
Gene ID(Mouse) 16173
Gene symbolIl18
UniProt ID(Mouse) P70380
The Mouse Interleukin-18 (IL-18) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-18. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.小鼠白介素18(IL-18)未包被的ELISA试剂盒包含预先匹配的抗体对,以及用于执行定量酶联免疫吸附测定(ELISA)的试剂,以检测和定量小鼠IL-18的蛋白质水平。需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA设计用于测量匹配的抗体对之间结合的靶标的量。将靶标特异性抗体包被在微孔板孔的底部,这是一整夜的过程。然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Target information 靶标信息The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.该基因编码的蛋白质是促炎细胞因子。该细胞因子可以诱导T细胞的IFN-γ产生。该细胞因子和IL12的组合已显示抑制IL4依赖性IgE和IgG1的产生,并增强B细胞IgG2a的产生。 IL-18结合蛋白可以与这种细胞因子特异性相互作用,从而对它的生物学活性产生负面影响。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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