2. 试剂
  3. K0622 Thermo 分子标记,DecaLabel DNA Labeling Kit/DECA标签DNA标记试剂盒

K0622 Thermo 分子标记,DecaLabel DNA Labeling Kit/DECA标签DNA标记试剂盒

2024-10-24
DecaLabel DNA Labeling Kit/DECA标签DNA标记试剂盒

货号： K0622

规格：30 reactions

价格：1362

产品类型：核酸标记

品牌：Thermo Fisher

Thermo Scientific DecaLabel DNA Labeling Kit is an advanced system for fast synthesis of radiolabeled DNA probes of high specific activity. It's based on the improved random-primed method developed by Feinberg and Vogelstein.The primary improvement over traditional random-primer kits involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. In addition, Klenow Fragment, exo- has been genetically engineered to remove exonuclease activity. The improved enzyme does not degrade the labeled probe during reaction and allows high labeling yields even from low template amounts. DNA fragments of any length can be uniformly labeled. Two labeling mixes are provided for flexibility in using either radiolabeled dATP or dCTP.

优点：

- High specific radioactivityof probes—greater than 1 x 109dpm/µg DNA- Fast—5 minutes at 37°C- High yieldswith Klenow Fragment, exo no degradation of a labeled probe during reaction- Flexible—suitable for either radiolabeled dATP or dCTP

数据：

图1.Effect of time, amount of template on the specific radioactivity of a DNA probe

Varying amounts of Lambda DNA/HindIII fragments were labeled with 50µCi [α-33P]-dCTP (2500Ci/mmol) according to the protocol supplied with the kit.

组成成分：

▪Klenow Fragment, exo-▪Decanucleotides in Reaction Buffer▪Mix A (-dATP)▪Mix C (-dCTP)▪dNTP Mix▪Control Template▪Water, nuclease-free▪Detailed Protocol

相关产品：

▪DecaLabel DNA Labeling Kit/DNA标记试剂盒（货号#K0622）▪Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒（货号#0651）▪Biotin Chromogenic Detection Kit/生物素显色检测试剂盒（货号#0661）

技术参数

产品应用 Generation of radiolabeled DNA probes for use in a variety of hybridization experiments: Southern and Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations. 生成放射标记DNA探针，用于Southern和Northern 印迹、菌落/斑块杂交、点/槽印迹和原位杂交等各种杂交实验。

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