EC0113 Thermo ,DH10B Competent Cells/DH10B 感受态细胞-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

EC0113 Thermo ,DH10B Competent Cells/DH10B 感受态细胞

2024-10-24

DH10B Competent Cells/DH10B 感受态细胞

货号：EC0113

规格：10 x 100 µL

价格：1402

产品类型：感受态细胞

品牌：Thermo Fisher

Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently. These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.• High transformation efficiency: >1x109cfu/µg pUC19 DNA• Suitable for routine and high-throughput cloning applications• Allows for blue/white colony screening• Convenient two reactions per vial packaging• S.O.C. Outgrowth medium includedApplicationsDH10B Competent Cells are suitable for variety of applications requiring high transformation efficiency:• Efficient DNA cloning both of prokaryotic and eukaryotic origin• Cloning of large plasmids and BACs• Ideal for generation of cDNA libraries using plasmid-derived vectors• Site directed mutagenesisGenotypeF–mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ (ara-leu)7697galU galKλ–rpsL(StrR)nupGNote: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542).

优点：

- High transformation efficiency: >1x109cfu/µg pUC19 DNA- Suitable for routine and high-throughput cloning applications- Allows for blue/white colony screening- Convenient two reactions per vial packaging- S.O.C. Outgrowth medium included

数据：

图1.Transformation efficiency of DH10B Competent CellsThermo Scientific DH10B competent cells provide high transformation efficiency. The transformation efficiencies of DH10B competent cells, NEB 10-beta competent E. coli, and E. cloni™ 10G cells by Lucigen were compared using pUC19 DNA and following the manufacturers’ recommended protocols. The transformation efficiency specification for DH10B competent cells is 1.0 x 109CFU/µg DNA (represented by the red dashed line). The values shown are the averages of five transformations.

组成成分：

▪DH10B Competent Cells▪pUC19 DNA (10 pg/µL)▪S.O.C. Medium

EC0113 Thermo ,DH10B Competent Cells/DH10B 感受态细胞

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