2. 试剂
  3. EC0114 Thermo BL21(DE3)感受态细胞,BL21(DE3) Competent Cells/BL21(DE3)感受态细胞

EC0114 Thermo BL21(DE3)感受态细胞,BL21(DE3) Competent Cells/BL21(DE3)感受态细胞

2024-10-24
BL21(DE3) Competent Cells/BL21(DE3)感受态细胞

货号：EC0114

规格：10 x 100 µL

价格：1185

产品类型：感受态细胞

品牌：Thermo Fisher

Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.ApplicationsBL21(DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins.GenotypeF–ompThsdSB(rB–, mB–)gal dcm(DE3)Note: Not suitable for maintenance of expression plasmids. Use DH10B or DH5α competent cells that do not carry the gene for T7 RNA polymerase for plasmid DNA propagation (Cat. Nos. EC0112, EC0113).

优点：

- High transformation efficiency: >1x107cfu/µg pUC19 DNA- Specifically constructed for high-level expression of recombinant proteins- Genetic markers that minimize RNA and protein degradation- Convenient two reactions per vial packaging- S.O.C. Outgrowth medium included

组成成分：

▪BL21(DE3) Competent Cells▪pUC19 DNA (10 pg/µL)▪ S.O.C. Medium

相关产品：

▪DH5αCompetent Cells for Subcloning/DH5α 感受态细胞用于亚克隆（货号#EC0111)

▪DH5α Competent Cells/DH5α感受态细胞（货号#EC0112）▪DH10B Competent Cells/DH10B感受态细胞（货号#EC0113）▪BL21(DE3) Competent Cells/BL21(DE3)感受态细胞（货号#EC0114）

技术参数
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