K1441 Thermo DNA甲基化,EpiJET DNA Methylation Analysis Kit (MspI/HpaII)/EpiJET DNA甲基化分析试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

K1441 Thermo DNA甲基化,EpiJET DNA Methylation Analysis Kit (MspI/HpaII)/EpiJET DNA甲基化分析试剂盒

2024-10-24

EpiJET DNA Methylation Analysis Kit (MspI/HpaII)/EpiJET DNA甲基化分析试剂盒

货号：K1441

规格：200 reactions

价格：1120

产品类型：表观试剂

品牌：Thermo Fisher

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.The Thermo Scientific EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the5'-CCGG-3'tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.NoteDNA sample quality is important for efficient digestion by restriction endonucleases or downstream applications. We recommend use of spin column based kits, such as the Thermo ScientificGeneJET Genomic DNA Purification Kit(#K0721, #K0722).

优点：

-Fast—complete digestion of genomic DNA in 1 hour at 37°C-Efficient—specially formulated enzymes for epigenetic studies of DNA methylation status

数据：

图1.Parallel digestion of methylated and unmethylated DNA with the restriction enzyme pair (MspI/HpaII)Methylated and unmethylated linearized plasmid DNA (pUC19/SmaI) were mixed with human blood genomic DNA and digested in parallel with methylation-sensitive Epi HpaII restriction enzyme and methylation insensitive isoschizomer Epi MspI for 1 hour at 37°C. Methylated plasmid DNA and naturally methylated gDNA were protected from cleavage with Epi HpaII, but were cleaved with insensitive Epi MspI. Unmethylated plasmid DNA was cleaved with both enzymes.1, 4 – undigested DNA (control)2, 5 – after digestion with Epi HpaII3, 6 – after digestion with Epi MspI

组成成分：

▪EpiMspl: 200 µL▪ Epi HpaII: 200 µL▪ 10X EpiBuffer: 1 mL▪ Control pUC19/SmaI DNA Unmethylated (0.5 µg/ µL): 20 µL▪ Control pUC19/SmaI DNA CpG Methylated (0.5 µg/ µL): 20 µL

相关产品：

▪EpiJET DNA Methylation Analysis Kit (MspI/HpaII)/EpiJET DNA甲基化分析试剂盒（货号#K1441）▪EpiJET Bisulfite Conversion Kit/EpiJET Bisulfite DNA转录试剂盒（货号#K1461）▪EpiJET 5-hmC and 5-mC Analysis Kit /EpiJET 5-hmC和 5-mC分析试剂盒（货号#K1501）▪Modification Reagent/修饰试剂（货号#R2101）▪Modification Solution I/修饰试剂I（货号#R2111）▪Modification Solution II/修饰试剂II（货号#R2121）▪Wash Buffer for Bisulfite Conversion Kit (concentrated)/Bisulfite DNA转录试剂盒清晰缓冲液（浓缩）（货号#R2141）

技术参数

产品优点 1. Fast—complete digestion of genomic DNA in 1 hour at 37°C 2. Efficient—specially formulated enzymes for epigenetic studies of DNA methylation status

产品应用 CpG methylation analysis at 5'-CCGG-3' loci

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K1441 Thermo DNA甲基化,EpiJET DNA Methylation Analysis Kit (MspI/HpaII)/EpiJET DNA甲基化分析试剂盒

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