Thermo Fisher Bpu10I内切酶 ER1181,Bpu10I (5 U/µL)/Bpu10I内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher Bpu10I内切酶 ER1181,Bpu10I (5 U/µL)/Bpu10I内切酶

2024-10-24

Bpu10I (5 U/µL)/Bpu10I内切酶

货号：ER1181

规格：200 units

价格：1054

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific Bpu10I restriction enzyme recognizes CCTNAGC(-5/-2)^ sites and cuts best at 37°C in its own unique buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:A large excess of enzyme (>4U/1 µg DNA) may result in incomplete DNA cleavage. Therefore, we recommend increasing the incubation time instead of using an excess of Bpu10I. Low salt, high glycerol (>5%) concentrations, pH > 7.0, or a large excess of enzyme may result in star activity. For cleavage with Bpu10I, at least two copies of its recognition sequence are required. For methylation sensitivity, refer to product specifications.Thermo Scientific Bpu10I限制性内切酶识别CCTNAGC（-5 / -2）^位点，并在其配套的缓冲液中于37°C切割效果最佳。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。

Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。此外，通用的Tango缓冲液为双消化提供了便利。在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：大量过量的酶（> 4 U / 1 µg DNA）可能会导致DNA切割不完全。因此，我们建议增加孵育时间，而不要使用过量的Bpu10I。低盐，高甘油（> 5％）浓度，pH> 7.0或酶大量过量可能导致星号活性。为了用Bpu10I切割，需要至少两个拷贝的其识别序列。有关甲基化敏感性，请参阅产品说明。

优点：

- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 卓越的质量—严格的质量控制和行业领先的生产工艺- 便利的颜色编码的五缓冲液系统- 包括用于双酶切的通用型 Tango 缓冲液- 在反应缓冲液中预混合 BSA

数据：

技术参数

产品优点 1. 卓越的质量—严格的质量控制和行业领先的生产工艺 2. 便利的颜色编码的五缓冲液系统 3. 包括用于双酶切的通用型 Tango 缓冲液 4. 在反应缓冲液中预混合 BSA 5. 多种特异性限制性核酸内切酶可供选择

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •单核苷酸多态性（SNP）

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Thermo Fisher Bpu10I内切酶 ER1181,Bpu10I (5 U/µL)/Bpu10I内切酶

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