Thermo Fisher DNA内切酶 DpnⅠ ER1701,DpnⅠ(MalⅠ) (10 U/µL) /内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher DNA内切酶 DpnⅠ ER1701,DpnⅠ(MalⅠ) (10 U/µL) /内切酶

2024-10-24

DpnⅠ(MalⅠ) (10 U/µL) /内切酶

货号：ER1701,ER1702,ER1705

规格：500 units,2500 units,1000 units

价格：446,1750,770

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA. For methylation sensitivity, refer to product specifications.Thermo Scientific DpnI 限制性内切酶可识别 Gm6A^TC 位点，于 37°C 下在 Tango 缓冲液中的切割效果最佳（同裂酶：MalI）。参见限制性内切酶的反应条件表格，了解该酶和其他限制性内切酶的酶活性、双重酶切条件和热灭活。备注：也可作为FastDigest 酶提供，用于快速 DNA 酶切。Thermo Scientific 常规限制性核酸内切酶是高质量限制性内切酶的大量汇集物，经过优化以便在五缓冲液系统的一种缓冲液中发挥作用。此外还提供通用 Tango 缓冲液，便于进行双酶切。所有酶在推荐的缓冲液和反应条件下均表现出 100% 活性。为确保一致的性能，Thermo Scientific 限制性内切酶反应缓冲液包含预混合 BSA，其可增强多种酶的稳定性，并与 DNA 制剂中可能存在的污染物相结合。注：DpnI 需要在识别序列中存在 N6-甲基腺嘌呤，才能切割 DNA。从 dam+ 菌株纯化的 DNA 将是 DpnI 的底物。DpnI 只能切割完全腺甲基化 dam 位点。半腺甲基化 dam 位点的 DpnI 切割速度慢60倍。DpnI、Bsp143I 和 MboI 均可识别相同的序列，但具有不同的甲基化敏感性和切割位点。使用 pBR322 DNA 进行了试验。无 CpG 甲基化敏感性、dam 甲基化敏感性，无 dcm甲基化敏感性。

优点：

- Superior quality—stringent quality control and industry leading manufacturing process- Convenient color-coded Five Buffer System- Includes universal Tango buffer for double-digestions- BSA premixed in reaction buffers- Wide selection of restriction endonuclease specificities- 卓越的质量—严格的质量控制和行业领先的生产工艺- 便利的颜色编码的五缓冲液系统- 包括用于双酶切的通用型 Tango 缓冲液- 在反应缓冲液中预混合 BSA- 多种特异性限制性核酸内切酶可供选择

数据：

技术参数

产品优点 1. 优异的质量—严格的质量控制和业内领先的生产流程 2. 使用颜色标记的五大便捷缓冲系统 3. 包含适于双酶切的通用型Tango缓冲液 4. 反应缓冲液中预先混入BSA 5. 种类齐全，特异性限制性内切酶的广泛选择

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •SNP

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Thermo Fisher DNA内切酶 DpnⅠ ER1701,DpnⅠ(MalⅠ) (10 U/µL) /内切酶

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