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  3. K0171 PCR Thermo ,PCR Master Mix (2X)

K0171 PCR Thermo ,PCR Master Mix (2X)

2024-10-24
PCR Master Mix (2X)

货号：K0171,K0172

规格：200 reactions,1000 reactions

价格：201,780

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific PCR Master Mix is a 2X concentrated solution ofTaqDNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.Note• The error rate ofTaqDNA Polymerase in PCR is 2.2 x 10-5errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

优点：

-Convenient, ready-to-use mix-Thermostable—half life is more than 40 min at 95°C-Generates PCR products with 3'-dA overhangs-Incorporates modified nucleotides(e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

组成成分：

▪4 x 1.25 mL PCR Master Mix (2X), which includes:— Taq DNA polymerase (0.05 U/µL),— reaction buffer,— 4 mM MgCl2, and— 0.4 mM of each dNTP▪ 4 x 1.25 mL Nuclease-free waterStore at -20°C.

相关产品：

▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化

技术参数

产品应用 • Routine PCR amplification of DNA fragments up to 5 kb • High throughput PCR • DNA labeling

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