DNase I, RNase-free (supplied with MnCl2) (1 U/µL)

货号：EN0525

规格：1000 units

价格：778

产品类型：酶及抑制剂

品牌：Thermo Fisher

描述Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.Highlights• Recombinant enzyme• Purified from non-animal host with a lower level of intrinsic RNasesApplications• Preparation of DNA-free RNA• Removal of template DNA following in vitro transcription• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR• DNA labeling by nick-translation in conjunction with DNA Polymerase I• Studies of DNA-protein interactions by DNase I, RNase-free footprinting• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is usedNoteDNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.DNase I activity in the presence of Mg2+ or Mn2+

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