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F566L PCR Thermo ,Phusion Green Hot Start II High-Fidelity PCR Master Mix/绿色热启动II高保真PCR混合液

2024-10-24

Phusion Green Hot Start II High-Fidelity PCR Master Mix/绿色热启动II高保真PCR混合液

货号：F566L,F566S

规格：500 reactions,100 reactions

价格：5627,1412

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that ofTaq, and 6-fold lower than that ofPfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.With Phusion Hot Start II High-Fidelity DNA Polymerases amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature. The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up. At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures so it does not require a separate activation step in PCR protocols.Phusion Green Hot Start II High-Fidelity PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

优点：

- Reaction set up at room temperature- No non-specific amplification and primer degradation during reaction set up- Zero-time reactivation due to unique hot start technology- High fidelity (52X Taq)- Fast PCR due to short extension times (15-30 s/kb)- Robust reactions, minimal optimization needed- Increased product yields with minimal enzyme amounts- Direct loading on gels

数据：

图1.Superior yields of long products20Êkb fragment from λ DNA and 7.5 kb fragment from human genomic DNA was amplified with different hot start high fidelity PCR master Mixes. Phusion Green Hot Start II PCR Master Mix was the only master mix capable of providing high amounts of desired PCR products. In contrast, master mixes from other suppliers delivered zero or significantly lower yields and in some cases non-specific products.

组成成分：

▪2X Phusion Green Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2in the final 1X concentration)▪ Water, nuclease-free▪ DMSOStore at -20°C.

F566L PCR Thermo ,Phusion Green Hot Start II High-Fidelity PCR Master Mix/绿色热启动II高保真PCR混合液

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