Taq DNA Polymerase, native, without BSA (5 U/µL)/Taq DNA聚合酶，天然，无BSA（5 U / µL）

货号：EP0282

规格：500 units

价格：389

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific NativeTaqDNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacteriumThermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. NativeTaqDNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found inE. coli.Highlights•Thermostable—half life is more than 40 min at 95°C.•Generates PCR products with 3'-dA overhangs.•Supplied with two buffers—10XTaqBuffer with KCl and 10XTaqBuffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.•Incorporates modified nucleotides(e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).Applications• Routine PCR amplification of DNA fragments up to 5 kb• DNA labeling (seeReference s 1-3)• High throughput PCR

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