Phusion Human Specimen Direct PCR Kit

货号：F150BID

规格：200 reactions

价格：2990

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples. A tiny amount of sample is used directly in the PCR reaction with no prior purification steps. This allows significant savings in both time and cost.Tested sample materials include buccal swabs, saliva, amniotic fluid, hair, fingernails, teeth, and skin biopsies. The kit is compatible with fresh, frozen, and even Formalin-Fixed, Paraffin Embedded (FFPE) human tissue samples. It includes optimized reagents and detailed protocols for many sample materials. The modified Phusion Hot Start II High-Fidelity DNA Polymerase utilized in the kit is a highly accurate proofreading DNA polymerase; PCR products obtained are perfectly suitable even for cloning purposes.Highlights• No need for time-consuming and expensive DNA purification steps• Two simple protocols for various applications• Extremely short PCR protocol times• Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific productThe optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that ofTaq-based DNA polymerases.Consistent results from various human samplesDifferent samples were placed directly into 20µL PCR reactions. A 237bp DNA fragment was successfully amplified using the control primers included in the kit. + and - denote control reactions with or without purified DNA.Significant time savings and superior performance in comparison to conventional PCR from purified DNAFour DNA fragments were amplified directly from buccal swabs using the dilution protocol. For the comparison reactions, DNA was first purified using a DNA purification kit from a major supplier followed by PCR with a hot start Taq DNA polymerase. Thermo Scientific Piko Thermal Cycler and UTW reaction vessels were used in PCR. Comparison of total cycling times shows that the Phusion Human Specimen Direct PCR Kit is 2 to 3 times faster allowing significant time savings in PCR.

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