Phire Animal Tissue Direct PCR Kit (without sampling tools)

货号：F140WH

规格：500 reactions

价格：3709

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects. The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized PCR reagents, Dilution buffer and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species. A detailed manual is provided that describes protocols when working with different types of tissues.Highlights• No need for time-consuming and expensive DNA purification steps• Very little sample material required• Two simple protocols for various applications•Phire Hot Start II DNA Polymeraseprovides high yields of specific product with short extension time (20 s/kb).The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that ofTaq-based polymerases.Genotyping F2 transgenic mice0.50 mm punches of mouse ear tissue were placed directly into 50µL PCR reactions. Two sets of primers were used in each reaction to genotype the 11 individual mice. The larger fragment was 490bp (transgenic) and the smaller fragment 250bp (wild type).Direct PCR from various animal tissuesSmall samples of various animal tissues were placed directly into 50µL PCR reactions. A specific DNA fragment was amplified with control primers. + and - denote control reactions with or without purified DNA.

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