Phire Plant Direct PCR Master Mix

货号：F160L,F160S

规格：1,250 reactions,250 reactions

价格：6774,1544

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols and others.Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria and yeast. The kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for amplification of plant DNA.Highlights• Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps.• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing• Specially engineered Phire Hot Start II DNA polymerase—extremely short PCR protocol times• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gelApplications• Genotyping• Transgene detection• Knockout analysis• SequencingThe optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.Robust amplification from variety of plantsAmplification of 0.5 kb DNA fragment directly from leaves (L) and seeds (S) from different plants. 0.5 mm punch of leaf or a small piece of crushed seed was placed directly into 50 µL of Phire Plant Direct PCR Master Mix. Same samples were amplified using a kit from other vendor according to supplier's instructions.M: O'GeneRuler Express DNA Ladder+: control reaction with purified DNAReliable results at wide range of amplicon lengthsDifferent length amplicons (0.5 kb, 1.5 kb and 5.0 kb) were amplified from dried Laurus leaves. 0.5 mm punches of leaves were placed directly into Phire Plant Direct PCR Master Mix.M: O'GeneRuler Express DNA LadderDilution & Storage protocol for re-testing1.5 kb DNA fragment from tobacco leaves was amplified with Phire Plant Direct PCR Master Mix using Direct protocol (D) or Dilution & Storage protocol (D&S). With D&S protocol samples were stored in Dilution Buffer for 1, 2 and 4 weeks at different temperatures: 1 - -20°C , 2 - +4°C, 3 — room temperature.M: O'GeneRuler Express DNA Ladder+: control reaction with purified tobacco DNA-: no-template control

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