Phire Tissue Direct PCR Master Mix

货号：F170L,F170S

规格：1,250 reactions ,250 reactions

价格：8072,1938

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.Highlights• Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing• Specially engineered Phire DNA polymerase—extremely short PCR protocol times• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gelApplications• Genotyping• Transgene detection• Knockout analysis• SequencingThe optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.Genotyping F2 transgenic mice0.50 mm punches of mouse ear tissue were placed directly into 50µL PCR reactions. Two sets of primers were used in each reaction to genotype the 11 individual mice. The larger fragment was 490bp (transgenic) and the smaller fragment 250bp (wild type).Reliable results in a wide range of amplicon lengthsDifferent length amplicons (237 bp, 2 kb, 7.5 kb) were successfully amplified directly from human saliva with Phire Tissue Direct PCR Master Mix.M – GeneRuler Express DNA LadderDilution & Storage protocol for re-testing237 bp DNA fragment from mouse ear was amplified with Phire Tissue Direct PCR Master Mix using Direct and Dilution & Storage protocols. The same fragment was amplified after samples were stored in Dilution Buffer for several weeks at +4°C.1 – Direct protocol, 2 – Dilution & Storage protocol, 3, 4, 5, 6 – Dilution & Storage protocol after one, two, three and four weeks storage in Dilution Buffer.M: O'GeneRuler Express DNA Ladder+: control reaction with purified mouse DNA-: no-template control.

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