Glycogen, molecular biology grade

货号：R0561

规格：R0561

价格：767

产品类型：DNA提取

品牌：Thermo Fisher

描述Thermo Scientific Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation, a visible pellet is formed, which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions with a higher efficiency than that of tRNA, linear polyacrylamide, or sonicated DNA (seeReferences 1-4).We offer two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery:•Glycogen, molecular biology grade, recommended for DNA precipitation (#R0561)•Glycogen, RNA grade, can be used for both RNA and DNA precipitation (#R0551)Highlights• Ideal for high recovery of oligonucleotides (> 8 bases) and low amounts of DNA/RNA (> 20 pg) from diluted solutions• Forms clearly visible pellets• Does not interfere with enzymatic reactions†• Does not interfere with gel electrophoresis of nucleic acids• Does not interfere with spectrophotometrical determination of DNA/RNA concentration, (A260 to 280measurements)• Compatible with DNA transformation andin vitrotransfectionFootnotes† At a final concentration up to 8 µg/ µL, Glycogen does not interfere with PCR, DNA sequencing, DNA digestion by endonucleases, ligation, cDNA synthesis, DNA labeling,in vitrotranscription, or bacterial transformation. At a final concentration up to 0.4 µg/ µL, Glycogen does not affectin vitrotransfection of eukaryotic cells with ExGen 500in vitroTransfection Reagent.Effect of Glycogen on Precipitation of Nucleic AcidsA 17nt DNA oligonucleotide was precipitated from 2pg/µL solution without glycogen and with 1µg/µL final concentration of Glycogen, molecular biology grade. A 200bp DNA fragment was precipitated from 50pg/µL solution without glycogen and with 0.05µg/µL final concentration of Glycogen, molecular biology grade. 100nt RNA transcript was precipitated from 50pg/µL solution, without Glycogen and with 0.05µg/µL final concentration of Glycogen, RNA grade. All precipitations were performed with 2.5X volumes of ethanol and 0.3M sodium acetate at room temperature for 5minutes. The concentration of the nucleic acids was measured spectrophotometrically.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48572.htm