Glycogen, RNA grade

货号：R0551

规格：2 x 0.1 mL

价格：1037

产品类型：DNA提取

品牌：Thermo Fisher

描述Thermo Scientific Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation, a visible pellet is formed which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions with a higher efficiency than that of tRNA, linear polyacrylamide, or sonicated DNA (seeReferences 1-4).We offer two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery:•Glycogen, RNA grade, can be used for both RNA and DNA precipitation (#R0551)•Glycogen, molecular biology grade, recommended for DNA precipitation (#R0561).Highlights• Ideal for high recovery of oligonucleotides (> 8 bases) and low amounts of DNA/RNA (> 20 pg) from diluted solutions• Forms clearly visible pellets• Does not interfere with enzymatic reactions†• Does not interfere with gel electrophoresis of nucleic acids• Does not interfere with spectrophotometrical determination of DNA/RNA concentration, (A260 to 280measurements)• Compatible with DNA transformation andin vitrotransfectionFootnote† – At a final concentration up to 8 µg/ µL, Glycogen does not interfere with PCR, DNA sequencing, DNA digestion by endonucleases, ligation, cDNA synthesis, DNA labeling,in vitrotranscription, or bacterial transformation. At a final concentration up to 0.4 µg/ µL, Glycogen does not affectin vitrotransfection of eukaryotic cells.

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