Ankyrin G Monoclonal Antibody (4G3F8)/锚蛋白G单克隆抗体（4G3F8）

货号：33-8800

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：A synthetic peptide derived from the spectrin-bind

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：4G3F8

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Assay Dependent(ELISA)；2-5 µg/mL(ICC)；2-5 µg/mL(IF)；Assay Dependent(IP)；1:1,000(WB)

靶标信息Ankyrin-G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin-G is also found at sites of epithelial cell-cell contact and in the intracellular membranes of some cells. Ankyrin-G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin-G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Ankyrin G Antibody (33-8800) in IFImmunofluorescence analysis of Ankyrin G was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ankyrin G (4G3F8) Mouse Monoclonal Antibody (Product # 33-8800) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Ankyrin G Antibody (33-8800) in ICCImmunofluorescence analysis of Ankyrin G was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Ankyrin G Monoclonal Antibody (4G3F8) (Product # 33-8800) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Plasma Membrane localization. Panel e represents SH-SY5Y with less expression on ANK G as compared to differentiated neurons. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Ankyrin G Antibody (33-8800)Scientific reports 2017 -Figure 2 The IKK-complex co-localizes with ankyrin-G to the axon initial segment. ( A - D ) Cortical neurons were cultured until maturity in vitro and were cytosol-extracted for the visualization of cytoskeletal-associated proteins. They were incubated for 5 minutes in 1% (m/V) Triton-X-100 (A-C) or 0.5% saponin (D) in 10 mM Na 3 PO 4 -buffer (pH 7.4), 1 mM MgCl 2 , 3 mM CaCl 2 , 150 mM NaCl. Extractions were performed at 4 degC, prior to fixation and immunostaining. Anti-IKKgamma/NEMO immunoreactivity is highlighted in red (A) or green (B), the AIS-specific marker 44 antibody 14D4 (green,A) or anti-AnkG (red, B) were used to identify the AIS (green). (C,D) Extractions were similarly performed followed by immunolabeling using pan-IKKalpha/beta - specific antibodies (red, rabbit polyclonal, H-470, SCBT) with the AIS labeled by anti-ankyrinG in green. Color overlays are depicted following background substraction and sharpening, arrows highlight AISs, scale bars, 10 mum. ( E ) IKKbeta-Flag and eGFP vectors were transfected into mouse cortical neurons on DIV13 using Ca-precipitation. 2 days following transfection, neurons were fixed and immunolabeled using a rabbit polyclonal anti-Flag-specific antibody (Sigma, red), as well as a mouse monoclonal ankyrin-G-specific antibody (Zymed). Note the Flag-tag-specific immunoreactivity within the ankyrin-G positive AIS stretch (arrow, yellow). ( F ) IKKbeta-Flag and ankG-eGFP vectors were transfected into mouse cortical neurons as above. 2

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参考文献：

1 Cell and tissue researchThe cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system.2 Cell and tissue researchThe cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system.3 eLifeKIF2A regulates the development of dentate granule cells and postnatal hippocampal wiring.4 Scientific reportsNF-κB regulates neuronal ankyrin-G via a negative feedback loop.5 eLifeKIF2A regulates the development of dentate granule cells and postnatal hippocampal wiring.

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