Tau Monoclonal Antibody (TAU-5)/Tau单克隆抗体（TAU-5）

货号：MA5-12808

规格：500 µL

价格：5548

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Purified bovine microtubule-associated proteins

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：TAU-5

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mLL	用法：	2-3 µg/mL(ICC)；2-3 µg/mL(IF)；1:100(IHC (P))；2 µg/mg protein lysate(IP)；1-2 µg/mL(WB)

产品详细信息MA5-12808 targets TAU in immunofluorescence, immunoprecipitation, and Western blot applications and shows reactivity with Bovine, Human, mouse, Ovine, and Rat samples.The MA5-12808 immunogen is purified bovine microtubule-associated proteins.靶标信息Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Tau Antibody (MA5-12808) in IFImmunofluorescence analysis of TAU was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TAU (TAU-5) Mouse Monoclonal Antibody (Product # MA5-12808) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. Tau Antibody (MA5-12808) in IHC (P)Immunohistochemistry analysis of TAU (TAU-5) showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a TAU Antibody (TAU-5) Mouse Monoclonal Antibody (Product # MA5-12808) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Tau Antibody (MA5-12808)Frontiers in neuroscience 2020 -Figure 1 Confocal microscopy analysis of untransfected human fibroblasts. Cells isolated from a skin biopsy showed a positive reactivity for Tau5 (cytoplasm), PHF (nucleus), and AT8 anti-serum (cytoplasm and nucleus). Nuclei were stained with Sytox (in red), while Tau was detected through FITC conjugated to the secondary antibody (in green). Sytox/FITC signal overlap is observed in yellow.

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参考文献：

1 Frontiers in neuroscienceOxidative Stress Modifies the Levels and Phosphorylation State of Tau Protein in Human Fibroblasts.2 Frontiers in neuroscienceOxidative Stress Modifies the Levels and Phosphorylation State of Tau Protein in Human Fibroblasts.3 Frontiers in molecular neuroscienceEffects ofAPOEGenotype on Brain Proteomic Network and Cell Type Changes in Alzheimer's Disease.4 Frontiers in cellular neuroscienceβ-Secretase 1's Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice.5 ProteomicsQuantitative Analysis of the Brain Ubiquitylome in Alzheimer's Disease.

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