mouse anti PCNA:FITC 伯乐 MCA1558F PCNA抗体

2024-10-29

mouse anti PCNA:FITC

货号:MCA1558F

规格:0.1 mg

市场价格:2933

产品类型:流式抗体

品牌:Bio-Rad

抗原:PCNA

物种:人/大鼠

宿主:小鼠

抗体亚型:IgG2a

克隆号:PC10

荧光染料:FITC

产品介绍
Mouse anti PCNA antibody, clone PC10recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction withFEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006).
产品详情

Target Species

Rat

Species Cross-ReactivityTarget SpeciesCross Reactivity
VertebratesExpected from Sequence
InvertebratesExpected from Sequence
Ferret
Chicken
Rabbit

Xenopus

Mouse
Horse
Sheep
Dog
Cat
Cynomolgus monkey
Rhesus Monkey
Hamster
Atlantic Salmon

Human

Bearded Dragon
Corn Snake
Nile Crocodile
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

ImmunogenRat PCNA made in the protein A expression vector pR1T2T
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry 1Neat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG2a Negative Control:FITCMCA1210FFlow0.1 mg
文献

1. Fenton, M.et al.(2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.Arterioscler Thromb Vasc Biol. 21: 220-6.2. Park, J.H.et al.(2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.J Comp Pathol. 139: 208-17.3. Izhak, L.et al.(2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.PLoS One. 7: e28305.4. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.Sci Adv. 2 (6): e1600708.5. Guzera, M.et al.(2016)In VitroInfluence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.PLoS One. 11 (5): e0154429.6. Khlebnikov, V. V.et al.(2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical StressNeuroscience and Behavioral Physiology. 46 (1): 100-5.7. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.J Clin Pediatr Dent. 42 (5): 391-7.8. Falbo, L.et al.(2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nat Commun. 11 (1): 1345.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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