| 产品详细信息Description: The monoclonal antibody CXCR3-173 recognizes mouse CD183 also known as CXCR3. CD183 is a seven transmembrane G-protein liked chemokine receptor which binds three ligands; CXCL9 (mig), CXCL10 (IP-10)and CXCL11 (ITAC). CD183 as been shown to play a role in CD4 T cell responses to grafts. CXCR3 knockout mice have compromised allograft rejection responses. Expression is found on NK cells, a subset of T lymphocytes and a subset of Tregs as well as preferential expression on Th1-polarizedcells.The antibody CXCR3-173 has been shown to affect chemotaxis in response to ligand. The presence of ligand eliminates staining with the antibody. In vivo addition of the antibody delays cardiac and pancreatic islet allograft rejection.Applications Reported: This CXCR3-173 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CXCR3-173 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene |
| 1.Clinical and translational allergyThe efficacy of oral and subcutaneous antigen-specific immunotherapy in murine cow's milk- and peanut allergy models."12-1831 was used in Flow cytometry/Cell sorting to compare oral immunotherapy and subcutaneous immunotherapy models for cows milk and peanut allergy."AuthorsVonk MM,Wagenaar L,Pieters RHH,Knippels LMJ,Willemsen LEM,Smit JJ,van Esch BCAM,Garssen J2.Frontiers in immunologyLimited Foxp3+Regulatory T Cells Response During AcuteTrypanosoma cruziInfection Is Required to Allow the Emergence of Robust Parasite-Specific CD8+T Cell Immunity."12-1831 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells during Trypanosoma cruzi infection."AuthorsAraujo Furlan CL,Tosello Boari J,Rodriguez C,Canale FP,Fiocca Vernengo F,Boccardo S,Beccaria CG,Adoue V,Joffre O,Gruppi A,Montes CL,Acosta Rodriguez EV3.Nature communicationsCD4+T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema."Published figure using CD183 (CXCR3) monoclonal antibody (Product # 12-1831-82) in Flow Cytometry"4.EBioMedicineT Cells Primed by Live Mycobacteria Versus a Tuberculosis Subunit Vaccine Exhibit Distinct Functional Properties."Published figure using CD183 (CXCR3) monoclonal antibody (Product # 12-1831-82) in Flow Cytometry"5.Cell metabolismCD38-NAD+Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response.AuthorsChatterjee S,Daenthanasanmak A,Chakraborty P,Wyatt MW,Dhar P,Selvam SP,Fu J,Zhang J,Nguyen H,Kang I,Toth K,Al-Homrani M,Husain M,Beeson G,Ball L,Helke K,Husain S,Garrett-Mayer E,Hardiman G,Mehrotra M,Nishimura MI,Beeson CC,Bupp MG,Wu J,Ogretmen B,Paulos CM,Rathmell J,Yu XZ,Mehrotra S |
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