Thermo,EZ-Link Desthiobiotinylation and Pull-Down Kit/EZ-Link脱硫生物素化和下拉试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo,EZ-Link Desthiobiotinylation and Pull-Down Kit/EZ-Link脱硫生物素化和下拉试剂盒

2024-10-24

EZ-Link Desthiobiotinylation and Pull-Down Kit/EZ-Link脱硫生物素化和下拉试剂盒

货号：16138

规格：5 reactions

价格：8911

产品类型：免疫印迹/组化

品牌：Thermo Fisher

The Thermo Scientific EZ-Link Desthiobiotinylation and Pull-Down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.This all-in-one labeling and purification kit uses Sulfo-NHS-LC-Desthiobiotin, an amine-reactive NHS-ester reagent, to effectively tag a purified or mixed protein sample for use as bait in capturing and purifying protein interactions. The desthiobiotin tags of labeled proteins bind to streptavidin with high specificity yet readily elute with very mild conditions (i.e., by competitive displacement with regular, free biotin). The system enables effective yet gentle capture and isolation (pull-down) of specific protein interaction complexes from cell lysates using streptavidin agarose beads. The complete kit includes ready-to-use 1 mg vials of desthiobiotinylation reagent, desalting columns to process labeled protein, and high-capacity streptavidin agarose with buffers to perform several microcentrifuge-scale pull-down assay experiments with ease. Identify novel interactions between a known protein (bait) and previously undiscovered target (prey), or confirm interactions among known bait and prey proteins.Desthiobiotinis a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (1/Kd = 1011vs. 1015M, respectively). Consequently, desthiobiotinylated bait proteins and their interacting partners can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down assay experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. The modified avidin-biotin affinity system also eliminates the need to use harsh elution conditions that might disassociate complexes and/or damage the target protein or cell. Desthiobiotin-based techniques are ideal when using native or recombinant proteins that are not expressed with a fusion tag and when isolating captured proteins under native conditions, such as targeting intact cells or cell surface proteins.Sulfo-NHS-LC-Desthiobiotin is a variant of biotin that is activated as a sulfo-NHS ester with a long-chain (LC) spacer arm to covalently label primary amines (-NH2) of proteins or other molecules with desthiobiotin groups. The No-Weigh Format (1 mg resealable vials) eliminates the difficulties associated with weighing small quantities of reagent and maximizes protection of unused reagent from hydrolysis.

优点和特点：

•Sulfo-NHS-LC-Desthiobiotin– label proteins with a biotin-like that specifically binds streptavidin but also easily elutes with mild conditions•Pull-down assay– simple, optimized protocol and reagents to label proteins and recover (purify) intact, functional protein interaction complexes•Complete kit– includes labeling reagent and desalting columns to prepare bait-proteins, and streptavidin agarose and elution buffer to perform pull-down

技术参数

组成成分 Sufficient For: 5 labeling reactions (0.2-5 mg protein each) and up to 40 pull-down experiments (50 µL each)• EZ-Link Sulfo-NHS-LC-Desthiobiotin, 2 x 1 mg• Zeba Spin Desalting Column (7K MWCO), 5 mL, 5 columns• High Capacity Streptavidin Agarose Resin, 2 mL• PBS Pack (makes 500mL), 1 pack• Biotin Elution Buffer, 8 mL

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48660.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Thermo,EZ-Link Desthiobiotinylation and Pull-Down Kit/EZ-Link脱硫生物素化和下拉试剂盒

相关推荐