Thermo,Pierce His Protein Interaction Pull-Down Kit/Pierce His蛋白相互作用下拉试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo,Pierce His Protein Interaction Pull-Down Kit/Pierce His蛋白相互作用下拉试剂盒

2024-10-24

Pierce His Protein Interaction Pull-Down Kit/Pierce His蛋白相互作用下拉试剂盒

货号：21277

规格：25 reactions

价格：6539

产品类型：免疫印迹/组化

品牌：Thermo Fisher

Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

优点和特点：

•6xHis pull-down (Product No. 21277)—purifies protein interactors of any His-tagged fusion protein•Complete kits—provide all components and detailed protocol for purifying protein:protein interactions•No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)•Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples•Flexible—instructions include protocols for use with bait and prey proteins expressed from a variety of sample types•Less non-specific binding—Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding•Binding—Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin

应用：

• Discover a new protein:protein interaction from a cell lysate• Confirm a putative interaction from a cell lysate or with a previously purified protein• Extract protein:protein interaction information fromin vitrotranscription/translation lysates

数据：

Detailed description of lanes in western blot figure

Procedure summary for GST Tag and His Tag Pull-Down KitsPull-down is a small-scale affinity purification technique similar to immunoprecipitation (IP), except that the antibody function of is replaced by some other affinity system. In this case, the affinity system is either a GST-tagged protein that can be captured by glutathione agarose beads or a His-tagged protein that can be captured by metal chelate (cobalt) agarose beads. The fusion-tagged protein acts as the bait to capture a putative binding partner (i.e., the prey). In a typical pull-down assay, the immobilized bait protein is incubated with a cell lysate. After the prescribed washing steps, the 'interactors are selectively eluted for analysis in-gel or by Western blot.

技术参数

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Thermo,Pierce His Protein Interaction Pull-Down Kit/Pierce His蛋白相互作用下拉试剂盒

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