IgM Monoclonal Antibody (SA-DA4), Biotin, eBioscience

货号：13-9998-82

规格：100 µg

市场价格：1132

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：SA-DA4

荧光染料：Biotin

类型:	人B细胞流抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience
浓度：	0.5 mg/mL	用法：	1μg/test(Flow)

产品详细信息Description:The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributedto both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: The SA-DA4 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining.Applications Tested: The SA-DA4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IgM Antibody (13-9998-82) in FlowStaining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.25 µg of Anti-Human IgM Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.PLoS biologyA new branched proximity hybridization assay for the quantification of nanoscale protein-protein proximity."Published figure using IgM monoclonal antibody (Product # 13-9998-82) in Immunohistochemistry"2.Frontiers in immunologySynthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells."Published figure using IgM monoclonal antibody (Product # 13-9998-82) in Flow Cytometry"3.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."Published figure using IgM monoclonal antibody (Product # 13-9998-82) in Flow Cytometry"

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