IgM Monoclonal Antibody (SA-DA4), eFluor 450, eBioscience

货号：48-9998-41,48-9998-42

规格：25 tests,100 tests

市场价格：1299,3202

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：SA-DA4

荧光染料：eFluor 450

类型:	人B细胞流抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450, eBioscience
浓度：	5μL/test	用法：	5μL(0.125μg)/test(Flow)

产品详细信息Description:The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IgM Antibody (48-9998-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0198-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human IgM eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."Published figure using IgM monoclonal antibody (Product # 48-9998-42) in Flow Cytometry"2.Frontiers in immunologySynthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells."Published figure using IgM monoclonal antibody (Product # 48-9998-42) in Flow Cytometry"

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