IgM Monoclonal Antibody (SA-DA4), eBioscience

货号：14-9998-82,14-9998-37,14-9998-95

规格：100 μg,2 mg,10 mg

市场价格：1615,11967,0

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：其它

类型:	人B细胞流抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	1 μg/test(Flow); Assay-Dependent(IHC(F)); Assay-Dependent(ELISA)

产品详细信息Description:The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.Applications Reported: The SA-DA4 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA. It has also been reported for use in affinity purification of human IgM.Applications Tested: The SA-DA4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IgM Antibody (14-9998-82) in FlowStaining of prelysed normal human peripheral blood cells with Anti-Human IgM Purified followed by Anti-Mouse IgG FITC.Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)B cell development in GALT: role of bacterial superantigen-like molecules."14-9998 was used in Immunofluorescence to provide direct evidence that B cell development in gut-associated lymphoid tissue (GLAT) may be driven by superantigen-like molecules, and that bacterial spores modulate host immunity."2.Frontiers in immunologySynthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells."Published figure using IgM monoclonal antibody (Product # 14-9998-82) in Flow Cytometry"3.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."Published figure using IgM monoclonal antibody (Product # 14-9998-82) in Flow Cytometry"4.Journal of immunology (Baltimore, Md. : 1950)CD20+ B cell depletion alters T cell homing."14-9998 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms of CD20 depletion in the treatment of autoimmune inflammatory disorders, showing that CD20+ B cell depletion alters T cell homing."

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48769.htm