HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780 Thermo 47-9956-41 HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780 Thermo 47-9956-41 HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780

2024-10-28

HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780

货号：47-9956-41,47-9956-42

规格：25 Tests,100 Tests

市场价格：1545,3163

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

荧光染料：APC-eFluor 780

抗体类型	一抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor 780
浓度：	5 µL/Test	用法：	5 μL (0.03 μg)/test (Flow)
产品详细信息Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.Applications Reported: This LN3 antibody has been reported for use in flowcytometric analysis.Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chaincontains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
数据
HLA-DR Antibody (47-9956-41) in FlowNormal human peripheral blood cells were stained with Mouse IgG2b kappa Isotype Control, APC-eFluor 780 (Product # 47-4732-80) (blue histogram) or HLA-DR Monoclonal Antibody, APC-eFluor 780 (purple histogram). Cells in the lymphocyte gate (left) or monocyte gate (right) were used for analysis.
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参考文献：
1.Stem cell research & therapyComprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta."Published figure using HLA-DR monoclonal antibody (Product # 47-9956-42) in Flow Cytometry"2.Stem cell reportsLack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors."Published figure using HLA-DR monoclonal antibody (Product # 47-9956-42) in Flow Cytometry"3.Frontiers in immunologyBovine Lactoferrin Enhances TLR7-Mediated Responses in Plasmacytoid Dendritic Cells in Elderly Women: Results From a Nutritional Intervention Study With Bovine Lactoferrin, GOS and Vitamin D."47-9956 was used in Flow cytometry/Cell sorting to investigate the potential to restore Toll like receptor responsiveness of plasmacytoid dendritic cells and myeloid dendritic cells, and to reduce inflammatory markers in serum."4.ImmunityAryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages."47-9956 was used in Flow cytometry/Cell sorting to elucidate the role of the aryl hydrocarbon receptor as a molecular switch for monocyte fate specification in response to micro-environmental factors."5.Cell reportsLarge-Scale Human Dendritic Cell Differentiation Revealing Notch-Dependent Lineage Bifurcation and Heterogeneity."Published figure using HLA-DR monoclonal antibody (Product # 47-9956-42) in Flow Cytometry"

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HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780 Thermo 47-9956-41 HLA-DR Monoclonal Antibody (LN3), APC-eFluor 780

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