Rat anti Mouse P2X7:Alexa Fluor® 488 BIO-RAD MCA4713A488 Rat anti Mouse P2X7:Alexa Fluor® 488-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Mouse P2X7:Alexa Fluor® 488 BIO-RAD MCA4713A488 Rat anti Mouse P2X7:Alexa Fluor® 488

2024-10-29

Rat anti Mouse P2X7:Alexa Fluor® 488

货号：MCA4713A488

规格：100 Tests/1ml

市场价格：3290

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：P2X7

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

荧光染料：Alexa Fluor 488

产品介绍
Rat anti Mouse P2X7 antibody, clone Hano43recognizes the P2X purinoceptor 7, also known as P2X7. The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic. It is a cation selective ion channel that opens up on binding of extracellular ATP. Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900 Da. Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP. Should ATP stimulation continue the cell will become irreversibly damaged and die. P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation. As such, P2X7 blockers might be useful as anti-inflammatory agents.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Alexa Fluor 488 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	A P2X7-expression construct and a final boost with P2X7-transfected HEK cells.
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Adriouch, S.et al.(2005) Probing the expression and function of the P2X7 purinoceptor with antibodies raised by genetic immunization.Cell Immunol. 236: 72-7.2.Adriouch, S.et al.(2008) ADP-ribosylation at R125 gates the P2X7 ion channel by presenting a covalent ligand to its nucleotide binding site.FASEB J. 2008 Mar;22(3):861-9.3.Schwarz, N.et al(2009) Activation of the P2X7 ion channel by soluble and covalently bound ligands.Purinergic Signal. 5: 139-49.4.Aswad, F. and Dennert, G. (2006) P2X7 receptor expression levels determine lethal effects of a purine based danger signal in T lymphocytes.Cell Immunol. 243: 58-65.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Rat anti Mouse P2X7:Alexa Fluor® 488 BIO-RAD MCA4713A488 Rat anti Mouse P2X7:Alexa Fluor® 488

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