CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE, eBioscience

货号：12-0629-42,12-0629-41

规格：100 Tests,25 Tests

市场价格：2271,925

产品类型：流式抗体

品牌：eBioscience

抗原：CD62L

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：DREG-56 (DREG56)

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.Applications Tested: The DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.仅用于科研。不用于诊断过程。

数据

CD62L (L-Selectin) Antibody (12-0629-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD62L (L-Selectin) PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Molecular & cellular proteomics : MCPFacile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers."12-0629 was used in Flow cytometry/Cell sorting to develop a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers, for future cancer biomarker discovery."AuthorsBing T,Shangguan D,Wang YYear2015SpeciesHuman 2. Journal of immunology (Baltimore, Md. : 1950)CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype."12-0629 was used in Flow cytometry/Cell sorting to demonstrate that CD55 is a potent costimulator and activator of human naive CD4(+) cells."AuthorsSutavani RV,Bradley RG,Ramage JM,Jackson AM,Durrant LG,Spendlove I3. Molecular medicine reportsn-butanol extract from Foliumisatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 12-0629-42) in Flow Cytometry"4. Scientific reportsImmune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue."12-0629 was used in Flow cytometry/Cell sorting to reveal that IL-1β and IL-17 had little effect on pro-fibrotic alterations but promote inflammation and metabolic dysfunction in adipose tissue."AuthorsCaër C,Rouault C,Le Roy T,Poitou C,Aron-Wisnewsky J,Torcivia A,Bichet JC,Clément K,Guerre-Millo M,André SYear2017SpeciesHuman 5. Molecular & cellular proteomics : MCPFacile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers."12-0629 was used in Flow cytometry/Cell sorting to develop a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers, for future cancer biomarker discovery."AuthorsBing T,Shangguan D,Wang Y

技术参数

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