CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-Cyanine5, eBioscience

货号：15-0629-42,15-0629-41

规格：100 Tests,25 Tests

市场价格：2677,1237

产品类型：流式抗体

品牌：eBioscience

抗原：CD62L

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：DREG-56 (DREG56)

荧光染料：PE-Cy5

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.Applications Tested: This DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.仅用于科研。不用于诊断过程。

数据

CD62L (L-Selectin) Antibody (15-0629-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD62L (L-Selectin) PE-Cyanine5 (filled histogram).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.British journal of pharmacologyInhibition of CXCR3-mediated chemotaxis by the human chemokine receptor-like protein CCX-CKR."15-0629 was used in Flow cytometry/Cell sorting to study the role of hCCX-CKR in hCXCR3-induced chemotaxis."AuthorsVinet J,van Zwam M,Dijkstra IM,Brouwer N,van Weering HR,Watts A,Meijer M,Fokkens MR,Kannan V,Verzijl D,Vischer HF,Smit MJ,Leurs R,Biber K,Boddeke HW2.Nature communicationsHeterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 15-0629-42) in Flow Cytometry"3.Molecular medicine reportsn-butanol extract from Foliumisatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils."Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 15-0629-42) in Flow Cytometry"

技术参数

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