CD314 (NKG2D) Monoclonal Antibody (1D11), PerCP-eFluor 710, eBioscience

货号：46-5878-42

规格：100 Tests

市场价格：3163

产品类型：流式抗体

品牌：eBioscience

抗原：CD314

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1D11

荧光染料：PerCP-eFluor™ 710

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: This 1D11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1D11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD314 (NKG2D) Antibody (46-5878-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a eFluor® 450 (Product # 48-0086-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD314 (NKG2D) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Nature communicationsRSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection."Published figure using CD8a monoclonal antibody (Product # 12-0088-42) in Flow Cytometry"2.Stem cell reportsDetection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models."Published figure using CD8a monoclonal antibody (Product # 12-0088-42) in Flow Cytometry"3.Stem cell reportsLack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors."Published figure using CD8a monoclonal antibody (Product # 12-0088-42) in Flow Cytometry"

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