CD314 (NKG2D) Monoclonal Antibody (1D11), eBioscience

货号：14-5878-82

规格：100 µg

市场价格：2325

产品类型：流式抗体

品牌：eBioscience

抗原：CD314

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1D11

荧光染料：其它

抗体类型:	流式抗体	同型对照：
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow）;Assay-Dependent(IHC(F));Assay-Dependent(IP）；Assay-Dependent（Neu）;Assay-Dependent(FN)

产品详细信息Description:The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in inhibition of ligand binding in in vitro assays. Please use Functional Grade Purified 1D11 (cat. 16-5878) in functional assays. (Fluorochrome-conjugated 1D11 is recommended for use in flow cytometric analysis.).Applications Tested: The 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD314 (NKG2D) Antibody (14-5878-82) in FlowStaining of normal human peripheral blood cells with 0.06 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.06 µg of Anti-Human CD314 (NKG2D) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchMonocytes and γδ T cells control the acute-phase response to intravenous zoledronate: insights from a phase IV safety trial."14-5878 was used in Flow cytometry/Cell sorting to investigate the acute-phase response of otherwise healthy individuals to first-time intravenous treatment with aminobisphosphonates zoledronate."2.Scientific reportsFunctional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production."14-5878 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of Vδ2 T cells in HCV-infected patients compared to healthy controls."3.Journal of leukocyte biologyHistone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression."14-5878 was used in Flow cytometry/Cell sorting to investigate the effect of histone deacetylase inhibitors on immune cells, showing that they can impair NK cell viability and effector functions."

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