IgM Monoclonal Antibody (eB121-15F9), PE,

货号：12-5890-82

规格：100 µg

市场价格：2028

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eB121-15F9

荧光染料：PE

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.Applications Reported: eB121-15F9 has been reported for usein flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs.Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (12-5890-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.03 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Mouse IgM PE (right). Total viable cells were used for analysis.

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参考文献

1. GastroenterologyIndoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.2. ImmunobiologyGeneration of a novel Cr2 gene allele by homologous recombination that abrogates production of Cr2 but is sufficient for expression of Cr1.3. European journal of immunologyA possible contribution of retinoids to regulation of fetal B lymphopoiesis.4. The Journal of clinical investigationHuman herpesvirus-encoded kinase induces B cell lymphomas in vivo.5. Nature communicationsNFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

技术参数

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