IgM Monoclonal Antibody (eB121-15F9), PE-Cyanine7,

货号：25-5890-80,25-5890-82

规格：25 ug,100 ug

市场价格：1554,2350

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eB121-15F9

荧光染料：PE-Cy7

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.Applications Reported: This eB121-15F9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eB121-15F9 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (25-5890-80) in FlowStaining of mouse bone marrow cells with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.25 µg of Anti-Mouse IgM PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品

二抗荧光二抗免疫组化一抗标签抗体

参考文献

1. Journal of immunological methodsStromal cell independent B cell development in vitro: generation and recovery of autoreactive clones.2. Nature communicationsNFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.3. GastroenterologyIndoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.4. Journal of immunological methodsStromal cell independent B cell development in vitro: generation and recovery of autoreactive clones.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48921.htm