IgM Monoclonal Antibody (II/41), PE,

货号：12-5790-81,12-5790-82,12-5790-83

规格：50 ug,100 ug,200 ug

市场价格：1038,2096,2844

产品类型：流式抗体

品牌：eBioscience

抗原：lgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：PE

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: The II/41 antibody has been reported for use inflow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (12-5790-81) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse IgM PE (right). Total viable cells were used for analysis.

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参考文献

1. CellInduction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires.2. Journal of immunology (Baltimore, Md. : 1950)Mechanism for pre-B cell loss in VH-mutant rabbits.3. AutophagyB cell autophagy mediates TLR7-dependent autoimmunity and inflammation.4. Frontiers in immunologyGrowth Hormone (GH) Deficient Mice With GHRH Gene Ablation Are Severely Deficient in Vaccine and Immune Responses AgainstStreptococcus pneumoniae.5. Cell regeneration (London, England)A protocol for generating induced T cells by reprogramming B cellsinvivo.

技术参数

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