IgM Monoclonal Antibody (II/41), PE-Cyanine7,

货号：25-5790-81,25-5790-82

规格：50 ug,100 ug

市场价格：1267,2112

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：II/41

荧光染料：PE-Cy7

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息Description: The II/41 monoclonal antibody reacts with the µ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.Applications Reported: This II/41 antibody has been reported for use inflow cytometric analysis.Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (25-5790-81) in FlowStaining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse IgM PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. The FEBS journalTET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.2. The EMBO journalThe mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells.3. European journal of immunologyThe expression of mouse CLEC-2 on leucocyte subsets varies according to their anatomical location and inflammatory state.4. mBioIgG Responses to Porins and Lipopolysaccharide within an Outer Membrane-Based Vaccine against NontyphoidalSalmonellaDevelop at Discordant Rates.5. Journal of immunology (Baltimore, Md. : 1950)ORMDL3 Facilitates the Survival of Splenic B Cells via an ATF6α-Endoplasmic Reticulum Stress-Beclin1 Autophagy Regulatory Pathway.

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