Ly-49H Monoclonal Antibody (3D10), Biotin, eBioscience
货号：13-5886-82
规格：100 µg
市场价格：1442
产品类型：流式抗体
品牌：eBioscience
抗原：Ly-49H
物种：小鼠
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：3D10
荧光染料：Biotin
| 类型: | 流式抗体 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience |
| 浓度： | 0.5 mg/mL | 用法： | 0.125 μg/test(Flow) |
产品详细信息Description:
The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. Ithas been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
| 数据 |
| Ly-49H Antibody (13-5886-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.25 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 0.06 µg of Anti-Mouse Ly-49H Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Alcoholism, clinical and experimental researchA dynamic flux in natural killer cell subsets as a function of the duration of alcohol ingestion."13-5886 was used in Flow cytometry/Cell sorting to investigate the effect of duration of alcohol ingestion on NK cell function, showing a dynamic flux in NK cell subsets as a function of ingestion."2.BloodMouse Ly49G2+ NK cells dominate early responses during both immune reconstitution and activation independently of MHC."13-5886 was used in Flow cytometry/Cell sorting to show that during reconstitution after HSCT and various activation stimuli, preferential expansion of the Ly49G2 receptor on NK cells occurs independently of MHC haplotype."3.Nature communicationsLoss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis."13-5886 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which loss of HIF1α in natural killer cells inhibits tumour growth." |
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