Ly-49H Monoclonal Antibody (3D10), FITC, eBioscience

货号：11-5886-82

规格：100 µg

市场价格：1920

产品类型：流式抗体

品牌：eBioscience

抗原：Ly-49H

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：3D10

荧光染料：FITC

类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience
浓度：	0.5 mg/mL	用法：	0.5 μg/test(Flow)

产品详细信息Description:

The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. Ithas been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells.Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D10 antibody has been tested by flow cytometric analyis of C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Ly-49H Antibody (11-5886-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (left) or 0.25 µg of Anti-Mouse Ly-49H FITC (right). Total viable cells were used for analysis.

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参考文献：

1.Nature immunologyEpigenetic control of innate and adaptive immune memory."11-5886 was used in Flow cytometry/Cell sorting to demonstrate that differentiating and 'memory' natural killer cells possess distinct chromatin accessibility states, and that their epigenetic profiles reveal a 'poised' regulatory programme at the memory stage."2.Cell reportsNon-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection."11-5886 was used in Flow cytometry/Cell sorting to study the role of individual ISGF3 components as regulators of the natural killer cell response to viral infection."3.PLoS pathogensTrypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells."11-5886 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality."

技术参数

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