Human anti Human cytokeratin 14 antibody BIO-RAD VMA00012 Human anti Human cytokeratin 14 antibody

2024-10-28

Human anti Human cytokeratin 14 antibody

货号:VMA00012

规格:100μl

市场价格:2916

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:cytokeratin 14

物种:人

宿主:其它

抗体亚型:其它

克隆号:AbD03759

荧光染料:Purified

产品介绍
Human anti Human cytokeratin 14 antibodyrecognizes human cytokeratin 14 (CK14), a 472 amino acid, acidic type I intermediate filament. The intermediate filaments are expressed as heterodimers in epithelial tissues. CK14 is expressed in the basal compartment of all stratified squamous epithelia and is co-expressed with cytokeratin 5 (CK5).Mutations in the cytokeratin 5 (KRT5) and cytokeratin 14 (KRT14) genes have been identified in patients with the skin disorder epidermolysis bullosa simplex (Coulombeet al.1991) and in other epidermal conditions including Naegeli-Franceschetti-Jadassohn syndrome and dermatopathia pigmentosa reticularis (Lugassyet al.2006).Human anti Human cytokeratin 14 antibody recognizes human cytokeratin 14 as a band of ~52 kDa in HeLa cell lysates by western blotting under reducing conditions.
产品详情
Target SpeciesHuman
Product FormA bivalent human recombinant Fab selected from the HuCAL GOLD phage display library, expressed inE. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
PreparationRecombinant human monoclonal antibody purified by metal chelate affinity chromatography
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogenRecombinant human cytokeratin 14
AcknowledgementsPrecisionAb is a trademark of Bio-Rad Laboratories.This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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产品类型 单克隆抗体

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