Rabbit F(ab')2 anti Mouse IgG antibody BIO-RAD STAR8D405GA Rabbit F(ab')2 anti Mouse IgG antibody

2024-10-28

Rabbit F(ab')2 anti Mouse IgG antibody

货号:STAR8D405GA

规格:0.1 mg

市场价格:1675

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:小鼠

宿主:兔

抗体亚型:IgG

荧光染料:DyLight 405

产品介绍
Rabbit F(ab')2anti Mouse IgG antibodyrecognizes all subclasses of mouse IgG. Cross reactivity with rat IgG is expected. Cross reactivity with human serum proteins has been minimised by solid phase adsorbtion.STAR8B is suitable as a bridge in a PAP / APAAP complex. Peroxidase anti-Peroxidase (PAP) complexes are formed by antibodies raised towards Horse Radish Peroxidase (HRP), binding to HRP molecules. Such complexes are used as signal amplification reagents in immunochemical staining techniques. It must be emphasised that the primary antibody must be raised in the same species as the antibodies used to form the PAP complex, so that a secondary "linker" antibody can be used to "bridge" the primary antibody to the PAP complex.
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to DyLight®405 - liquid
PreparationPurified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion of the IgG followed by a gel filtration step to remove any remaining intact IgG or Fc fragments
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
ImmunogenWhole mouse IgG.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Dylight®405400420
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Seehafer, S.S.et al.(2011) Immunosuppression alters disease severity in juvenile Batten disease mice.J Neuroimmunol. 230 (1-2): 169-72.2.Motallebzadeh, R.et al.(2012) Blocking lymphotoxin signaling abrogates the development of ectopic lymphoid tissue within cardiac allografts and inhibits effector antibody responses.FASEB J. 26 (1): 51-62.3.Niven, J.et al.(2015) S100B Up-Regulates Macrophage Production of IL1β and CCL22 and Influences Severity of Retinal Inflammation.PLoS One. 10 (7): e0132688.

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