Recombinant Human WNT-1 BIO-RAD PHP183 Recombinant Human WNT-1

2024-10-29

Recombinant Human WNT-1

货号:PHP183

规格:10 µg

市场价格:3162

产品类型:细胞因子添加物

品牌:Bio-Rad

产品介绍
Wnt-1, formerly known as int-1, is a non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
PreparationPurified recombinant Wnt-1 expressed inE.coli
ReconstitutionReconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution10mM Sodium Phosphate, pH6.8
ActivityThe ED50was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Preservative StabilisersNone present
Protein Molecular Weight38.4 kDa (343 amino acid residues)
Purity>98% by SDS PAGE and HPLC
RegulatoryFor research purposes only
Guarantee3 months from date of reconstitution
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 FN

产品类型 重组蛋白

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