Mouse anti Human CD14 antibody, clone UCHM1 BIO-RAD MCA596A700 Mouse anti Human CD14 antibody, clone UCHM1

2024-10-29

Mouse anti Human CD14 antibody, clone UCHM1

货号:MCA596A700

规格:100 Tests/1ml

市场价格:3188

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD14

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:UCHM1

荧光染料:Alexa Fluor 700

产品介绍
Mouse anti Human CD14 antibody, clone UCHM1recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative (Hogget al.1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Alexa Fluor 700 - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Fusion PartnersSpleen cells from immunized BALB/c mice were fused with cells from the NS1-Ag4/1 mouse myeloma line.
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®700702723
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Hogg, N. & Horton, M.A. (1987) Myeloid antigens: new and previously defined clustersin Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J.,et al.2.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3.Jonker, M.et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.Leucocyte Typing IV. Oxford University Press p 1058-63.4.Hogg, N.et al.(1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.Immunology. 53 (4): 753-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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