Mouse anti Pig CD8 beta chain, clone PPT23 BIO-RAD MCA5954F Mouse anti Pig CD8 beta chain, clone PPT23-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Pig CD8 beta chain, clone PPT23 BIO-RAD MCA5954F Mouse anti Pig CD8 beta chain, clone PPT23

2024-10-29

Mouse anti Pig CD8 beta chain, clone PPT23

货号：MCA5954F

规格：0.1 mg

市场价格：3766

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD8 beta

物种：pig

宿主：小鼠

抗体亚型：IgG1

克隆号：PPT23

荧光染料：FITC

产品介绍
Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hiand as such defines this antibody as a specific marker of porcine α/β T cells, in thymic tissue both CD8loand CD8hicells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22.
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	Porcine thymus membrane lysate
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.Immunology. 92 (1): 45-52.2.Mair, K.H.et al.(2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS®MLV.Vet Microbiol. 176 (3-4): 352-7.3.Sinkora, M.et al.(2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.J Immunol. 178 (2): 711-9.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Pig CD8 beta chain, clone PPT23 BIO-RAD MCA5954F Mouse anti Pig CD8 beta chain, clone PPT23

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