Mouse anti Bovine Interferon Gamma:FITCBIO-RAD MCA1783F Mouse anti Bovine Interferon Gamma:FITC

2024-10-29

Mouse anti Bovine Interferon Gamma:FITC

货号:MCA1783F

规格:0.1 mg

市场价格:3783

产品类型:流式抗体

品牌:Bio-Rad

抗原:Interferon Gamma

物种:Bovine

宿主:小鼠

抗体亚型:IgG1

克隆号:CC302

荧光染料:FITC

产品介绍
Mouse anti Bovine IFNγ antibody, clone CC302recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodeset al.2000).Mouse anti bovine γ interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersenet al.2002). Mouse anti Bovine IFNγ antibody, clone CC302 has been used successfully for the evaluation of γ interferon levels in the sera of calves naturally infected withM. avium.subspparatuberculosis(Appanaet al.2013) as a detection reagent using an ELISA.
产品详情
Target SpeciesBovine
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Species Cross-ReactivityTarget SpeciesCross Reactivity
Human
Dog
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Cameron, C.M.et al.(2005) Myxoma virus M141R expresses a viral CD200 (vOX-2) that is responsible for down-regulation of macrophage and T-cell activation in vivo.J Virol. 79: 6052-67.2.de Mestre, A.et al.(2010) Split immunological tolerance to trophoblast.Int J Dev Biol. 54: 445-55.3.Elhmouzi-Younes, J.et al.(2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.Vet Res. 40: 54.4.Ferret-Bernard, S.et al.(2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.PLoS One. 5: e13705.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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