Mouse anti Human CD54:RPE-Alexa Fluor® 647 MCA1615P647 Mouse anti Human CD54:RPE-Alexa Fluor® 647-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD54:RPE-Alexa Fluor® 647 MCA1615P647 Mouse anti Human CD54:RPE-Alexa Fluor® 647

2024-10-29

Mouse anti Human CD54:RPE-Alexa Fluor® 647

货号：MCA1615P647

规格：100 Tests

市场价格：3162

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD54

物种：其它

宿主：小鼠

抗体亚型：IgG1

荧光染料：Alexa Fluor 647

产品介绍
Mouse anti Human CD54 antibody, clone 15.2recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor.CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains.Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendtet al.1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005).
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Reconstitution	Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution	Phosphate buffered saline
Immunogen	Human monocytes
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE-Alexa Fluor®647 488nm laser	496	667
Acknowledgements	This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light.Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647	MCA928P647	F	100 Tests/1ml
文献
1.Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.Edited by Knapp, W.et al. Oxford University Press pp 694-697.2.Holmes, C.H.et al.(1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.J Immunol. 144 (8): 3099-105.3.O'Brien, D.P.et al.(2009) Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.J Biol Chem. 283: 23922-30.4.Yang, P.et al.(2009) Expression and modulation of RPE cell membrane complement regulatory proteins.Invest Ophthalmol Vis Sci. 50: 3473-81.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Human CD54:RPE-Alexa Fluor® 647 MCA1615P647 Mouse anti Human CD54:RPE-Alexa Fluor® 647

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