Mouse anti Human p53 antibody, clone DO-1 伯乐 MCA1701 p53抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human p53 antibody, clone DO-1 伯乐 MCA1701 p53抗体

2024-10-29

Mouse anti Human p53 antibody, clone DO-1

货号：MCA1701

规格：0.1 mg

市场价格：2576

产品类型：流式抗体

品牌：Bio-Rad

抗原：p53

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：DO-1

荧光染料：Purified

产品介绍
Mouse anti Human p53 antibody, clone DO-1recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kernet al.1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Hauptet al.2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007)Mouse anti Human p53 antibody, clone DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25,common to isoforms 1-3 of p53.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mouse	x
Rat	x
Bovine	√
Cat	√
Horse	√
Green Monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Recombinant human p53.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Immunofluorescence	√
Immunohistology - Frozen	√
Immunohistology - Paraffin	√		1/25
Immunoprecipitation	√
Western Blotting	√		1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Clickhereto learn how we validate our PrecisionAb range.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Histology Positive Control TissueColon or breast carcinoma
数据
Western blot analysis of p53 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Mouse anti p53 antibody, clone DO-1 (MCA1701) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 11 second and (B) 30 second exposure
文献
1. Sironi, G.et al.(1999) p53 protein expression in conjunctival squamous cell carcinomas of domestic animals.Vet Ophthalmol. 2 (4): 227-231.2. Vojtěsek Bet al.(1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.J Immunol Methods. 151 (1-2): 237-44.3. Levesque, M.A.et al.(1995) Time-resolved immunofluorometric assay of p53 protein.Clin Chem. 41 (12 Pt 1): 1720-9.4. Carvalho, T.et al.(2009) Immunohistochemical evaluation of vascular urinary bladder tumors from cows with enzootic hematuria.Vet Pathol. 46 (2): 211-21.5. Phillips, A.et al.(2010) HDMX-L is expressed from a functional p53-responsive promoter in the first intron of the HDMX gene and participates in an autoregulatory feedback loop to control p53 activity.J Biol Chem. 285 (38): 29111-27.6. Bergman, L.M.et al.(2009) CtBPs promote cell survival through the maintenance of mitotic fidelity.Mol Cell Biol. 29: 4539-51.7. Hietanen, S.et al.(2000) Activation of p53 in cervical carcinoma cells by small molecules.Proc Natl Acad Sci U S A. 97 (15): 8501-6.8. Phelps, M.et al.(2003) p53-independent activation of the hdm2-P2 promoter through multiple transcription factor response elements results in elevated hdm2 expression in estrogen receptor alpha-positive breast cancer cells.Cancer Res. 63: 2616-23.

技术参数

产品应用 ELISA;IHC.WB;IP

产品类型 Monoclonal Antibody

推荐用于 ELISA;IHC.WB;IP

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Mouse anti Human p53 antibody, clone DO-1 伯乐 MCA1701 p53抗体

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