Mouse anti p53 antibody, clone DO-7 伯乐 MCA1703 CD45抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti p53 antibody, clone DO-7 伯乐 MCA1703 CD45抗体

2024-10-29

Mouse anti p53 antibody, clone DO-7

货号：MCA1703

规格：0.1 mg

市场价格：2576

产品类型：流式抗体

品牌：Bio-Rad

抗原：p53

物种：人

宿主：小鼠

抗体亚型：IgG2b

克隆号：DO-7

荧光染料：Purified

产品介绍
Mouse anti p53 antibody, clone DO-7recognizes the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization domain and a terminal regulatory domain (Joergeret al.2010).p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis (Hauptet al.2003) and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals (Takagiet al.2005). Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and 14-3-3σ, DNA repair and potentially apoptosis through interaction with Bax, Apaf-1, PUMA and NoxA (Thakuret al.2010). p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent system (Moll and Petrenko 2003)Mouse anti p53 antibody, clone DO-7 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multipleisoformslacking the N-teminal region.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mouse	x
Rat	x
Bovine	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Recombinant human p53.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin²	√		1/1000
Flow Cytometry ¹	√	1/50	1/100
Immunohistology - Frozen	√
Immunoprecipitation	√
Western Blotting	√		1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. Histology Positive Control TissueColon or breast carcinoma
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2b Negative Control	MCA691	Flow	100 Tests
文献
1.Bonsing, B. A.et al(1997) Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry.Cytometry. 28:11-24.2. Vojtĕsek, B.et al.(1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.J Immunol Methods. 151 (1-2): 237-44.3. Xinarianos, G.et al.(2002) p53 status correlates with the differential expression of the DNA mismatch repair protein MSH2 in non-small cell lung carcinoma.Int J Cancer. 101: 248-52.4. Huang, H.Y.et al.(2008) Immunohistochemical and biogenetic features of diffuse-type tenosynovial giant cell tumors: the potential roles of cyclin A, P53, and deletion of 15q in sarcomatous transformation.Clin Cancer Res. 14: 6023-32.5. Iannone, F.et al.(2005) Increased Bcl-2/p53 ratio in human osteoarthritic cartilage: a possible role in regulation of chondrocyte metabolism.Ann Rheum Dis. 64: 217-21.6. Lin, L.C.et al.(2006) p53 and p27 as predictors of clinical outcome for rectal-cancer patients receiving neoadjuvant therapy.Surg Oncol. 15: 211-6.

技术参数

产品应用 Flow;IHC;WB;IP

产品类型 Monoclonal Antibody

推荐用于 Flow;IHC;WB;IP

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Mouse anti p53 antibody, clone DO-7 伯乐 MCA1703 CD45抗体

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