Mouse anti PCNA antibody, clone PC10 伯乐 MCA1558 PCNA 抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti PCNA antibody, clone PC10 伯乐 MCA1558 PCNA 抗体

2024-10-29

Mouse anti PCNA antibody, clone PC10

货号：MCA1558

规格：0.1 mg

市场价格：2576

产品类型：流式抗体

品牌：Bio-Rad

抗原：PCNA

物种：大鼠

宿主：小鼠

抗体亚型：IgG2a

克隆号：PC10

荧光染料：Purified

产品介绍
Mouse anti PCNA antibody, clone PC10recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Liet al.1995) through its interaction withFEN1(Wuet al.1996). PCNA is the auxilliary protein for DNA polymerase δ (Bravoet al.1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Parket al.2008), chicken (Franz-Odendaal 2008) and abalone (Harriset al.2006).
产品详情
Target Species	Rat
Species Cross-Reactivity	Target Species	Cross Reactivity
Vertebrates	Expected from Sequence
Invertebrates	Expected from Sequence
Ferret	√
Chicken	√
Rabbit	√
Xenopus	√
Mouse	√
Horse	√
Sheep	√
Dog	√
Cat	√
Cynomolgus monkey	√
Rhesus Monkey	√
Hamster	√
Atlantic Salmon	√
Human	√
Bearded Dragon	√
Corn Snake	√
Nile Crocodile	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Carrier Free	Yes
Immunogen	Rat PCNA made in the protein A expression vector pR1T2T
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1	√	Neat	1/100
Immunohistology - Frozen
Immunohistology - Paraffin		1/50	1/100
Immunoprecipitation
Western Blotting			1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul. ImmunohistologyThis product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat pre-treatment prior to staining of paraffin sections. Histology Positive Control TissueTonsil
相关产品
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control	MCA1210	Flow	100 Tests
数据
Multiplex staining ofHeLawhole cell lysate with Mouse anti PCNA antibody, clone PC10 (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight 680 (STAR117D680GA) shown in red and Rabbit anti GAPDH(AHP1628)detected with Sheep anti Rabbit IgG:DyLight 488 shown in green.
文献
1. Fenton, M.et al.(2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.Arterioscler Thromb Vasc Biol. 21: 220-6.2. Park, J.H.et al.(2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.J Comp Pathol. 139: 208-17.3. Izhak, L.et al.(2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.PLoS One. 7: e28305.4. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.Sci Adv. 2 (6): e1600708.5. Guzera, M.et al.(2016)In VitroInfluence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.PLoS One. 11 (5): e0154429.6. Khlebnikov, V. V.et al.(2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical StressNeuroscience and Behavioral Physiology. 46 (1): 100-5.7. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.J Clin Pediatr Dent. 42 (5): 391-7.8. Falbo, L.et al.(2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.Nat Commun. 11 (1): 1345.

技术参数

产品应用 IP;IHC;Flow;WB

产品类型 Monoclonal Antibody

推荐用于 IP;IHC;Flow;WB

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Mouse anti PCNA antibody, clone PC10 伯乐 MCA1558 PCNA 抗体

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