| 产品介绍 | | |
| Mouse anti Human CD10 antibody, clone SN5c recognizes human neprilysin, also known as CD10, atriopeptidase, enkephalinase, neutral endopeptidase 24.11, skin fibroblast elastase or common acute lymphocytic leukemia antigen (CALLA). CD10 is a 749 aminoacid, ~100 kDa single pass type II transmembrane glycoprotein expressed by acute lymphoblastic leukaemia cells and by peripheral blood granulocytes.Defects in the MME gene encoding CD10 can lead to the development of the peripheral nervous system disorder, Charcot-Marie-Tooth disease 2T (CMY2T), an axonal form of Marie-Charcot-Tooth disease characterized by either dominantly inherited primary peripheral demyelinating neuropathies, designated CMT1, or primary peripheral axonal neuropathies showing axonal degeneration in the absence of any obvious myelin alteration (CMT2). |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | Pack Size: 0.1 mg 0.09% Sodium Azide Pack Size: 0.2 mg 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
| Immunogen | Partially purified cell membrane antigens from fresh leukemia cells |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse PS/NS1/1-Ag4-1 myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/100Pack Size: 0.1 mg1/10Pack Size: 0.2 mg | 1/200Pack Size: 0.1 mg1/25Pack Size: 0.2 mg |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| Western Blotting | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | Flow | 100 Tests |
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| Human Seroblock | BUF070A | Flow | 50 Tests |
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| 数据 |
| Figure A. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Rat IgG2a (MCA6005PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A)for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Mouse anti Human IL-2 (MCA1553PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer |
| 文献 |
| 1.Biddle, W.C.et al.(1989)In vitroandin vivocytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.Leuk Res. 13 (8): 699-707.2. Riemann, D.et al.(2001) Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains.Biochem J. 354 (Pt 1): 47-55.3. Diaz-Romero, J.et al.(2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflectbona fidededifferentiation rather than amplification of progenitor cells.J Cell Physiol. 214: 75-83.4. Pilling, D.et al.(2009) Identification of markers that distinguish monocyte-derived fibrocytes from monocytes, macrophages, and fibroblasts.PLoS One. 4 (10): e7475.5. Manini, I.et al.(2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print].6. Glynn, E. & Fromm, J.R. (2020) Immunophenotypic Characterization and Purification of Neoplastic Cells from Lymph Nodes Involved by T-Cell/Histiocyte-rich Large B-cell Lymphoma by Flow Cytometry and Flow Cytometric Cell Sorting.Cytometry B Clin Cytom. 98 (1): 88-98.7. Caponnetto, F.et al.(2020) Human Adipose-Derived Stem Cells in Madelung's Disease: Morphological and Functional Characterization.Cells. 10 (1): 44.8. Kirolos, S.A. and Gomer, R.H. (2022) The extracellular sialidase NEU3 induces neutrophil primingbioRxiv. 24 Feb [Epub ahead of print]. |
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